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The substrate Option was then saved at ?20°C until eventually the ammonium and nitrate contents were analyzed While using the San++ Constant-Flow Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate perseverance was according to a reaction with hydrazinium sulfate, sulfanilamide and N-(1-naphthyl) ethylenediamine dihydrochloride which end

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